alpha-Keratin 5/8 Antikörper

Antigen: alpha-Keratin 5/8

Klonalität: Monoklonal (C50)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN306823

Produktbeschreibung

Weitere Bezeichnung: alpha-Keratin 5 & 8

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: C50

Beschreibung: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.Simple epithelia express cytokeratin 8 in combination with 18. Basal cells of stratified epithelia express cytokeratin 5 with cytokeratin 14. Therefore this antibody reacts with a wide range of epithelia and their carcinomas.

Anwendungen

Applikationshinweise: Detects Keratin 5 & 8 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 52.5 kDa band, corresponding to Keratin 8.

Konzentration: 1 mg/ml

Lagerung: Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Beschränkungen: Nur für Forschungszwecke einsetzbar