V5 Epitope Tag Antikörper
| Antigen |
|
| Klonalität |
Monoklonal (SV5-Pk1) |
|
Wirt
|
|
|
Reaktivität
|
|
|
Applikation
|
 Alternativen Western Blot (WB), Immunpräzipitation (IP), ELISA, Immunhistochemie (Gefrierschnitte) (IHC (fro)), Radioimmunoassay (RIA)
Western Blot (WB) (99), ELISA (54), Immunfluoreszenz (IF) (50), Immunpräzipitation (IP) (36), Enzyme Immunoassay (EIA) (32), Durchflusszytometrie (FACS) (20), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (17), Immunhistochemie (IHC) (11), Immunzytochemie (ICC) (9), Immunhistochemie (Paraffinschnitte) (IHC (p)) (6), Radioimmunoassay (RIA) (5), Dot Blot (Dot) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
| Produktnummer |
ABIN304867 |
| Menge |
1000 µg (1 mg/ml) |
| Preis |
583,33 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 10 bis 15 Werktagen |
|
Weitere Bezeichnung
|
V5 Tag
|
|
Immunogen
|
Virus, Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
|
|
Isotyp
|
IgG2a (Passende Sekundärantikörper)
|
|
Klon
|
SV5-Pk1
|
|
Beschreibung
|
Gene: V5 Tag
|
|
Spezifität
|
Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr
|
|
Applikationshinweise
|
ELISA (1:1000 - 1:5000), IHC-Fr, IP, RIA, WB (1:1000 - 1:5000)
|
|
Konzentration
|
1 mg/ml
|
|
Reinigung
|
Affinity Chromatography
|
|
Buffer
|
0.09% sodium azide.
|
|
Lagerung
|
+4°C or -20°C, Avoid repeated freezing and thawing.
|
|
Forschungsgebiet
|
Tags/Labels
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
Alternativen zu Antigen "V5 Epitope Tag", Typ "Antikörper" finden
|
Wirte
|
Kaninchen (56), Maus (50), Ziege (18), Huhn (9), Schaf (1)
|
|
Reaktivitäten
|
Tag (16), Fusion Protein (Tag) (14), Alle Spezies (8), Virus (2)
|
|
Applikationen
|
Western Blot (WB) (99), ELISA (54), Immunfluoreszenz (IF) (50), Immunpräzipitation (IP) (36), Enzyme Immunoassay (EIA) (32), Durchflusszytometrie (FACS) (20), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (17), Immunhistochemie (IHC) (11), Immunzytochemie (ICC) (9), Immunhistochemie (Paraffinschnitte) (IHC (p)) (6), Radioimmunoassay (RIA) (5), Dot Blot (Dot) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Konjugate
|
HRP (15), FITC (13), Biotin (7), Agarose Beads (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitope
|
GKPIPNPLLGLDST (36)
|
