In den Warenkorb
Bestellen Sie auch über:
+49 (0)241 95 163 153
+49 (0)241 95 163 155

Nestin (NES) (AA 1484-1500) Antikörper

Antigen

Nestin (NES)
Synonyme FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin
Epitope
Alternativen

AA 1484-1500
Klonalität Polyklonal
Wirt
Alternativen

Kaninchen
Reaktivität
Alternativen

Rind (Kuh)
Applikation
Alternativen Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Produktnummer ABIN303340
Menge 50 µg  (1.0 mg/ml)
Preis 360,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung
Anwendungen Alternativen Sekundärantikörper zurück nach oben

Produktmerkmale Synonyms: NES, Nbla00170
Weitere Bezeichnung Nestin
Swiss-Prot P48681
Immunogen Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin.
Kreuzreaktivität Rind (Kuh), Hund, Hamster, Human, Maus, Kaninchen, Ratte (Rattus)
Beschreibung Nestin, is a large intermediate filament protein (class Type VI) that was first identified witha monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantlyin stem cells of the central nervous system in the neural tube. Nestin is expressed duringdevelopment and in myotendinous and neuromuscular junctions. Nestin expression isrestricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronalmarker, however, recent studies have found nestin expression in other cell types such asendothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes)and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced byneurofilaments.
Spezifität This antibody Recognizes Nestin. Species Reactivity: Tested: Human. Expected from sequence similarity: Bovine, Dog, Hamster, Macaque, Mouse, Rabbit, Rat

Anwendungen
Alternativen Sekundärantikörper zurück nach oben

Applikationshinweise ELISA (1/20000-1/80000). Immunofluorescence. Western Blot (1/500-1/3000). Immunohistochemistry on Paraffin Sections (5 µg/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Konzentration 1.0 mg/ml
Reinigung Aff - Purified
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.
Lagerung Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen
Anwendungen Sekundärantikörper zurück nach oben

Alternativen zu Antigen "Nestin (NES)", Typ "Antikörper" finden
Wirte Kaninchen (80), Maus (30), Huhn (3), Ziege (1)
Reaktivitäten Human (86), Maus (36), Ratte (Rattus) (32), Rind (Kuh) (6), Kaninchen (6), Hund (5), Pferd (4), Schwein (4), Huhn (1), Schimpanse (1), Hamster (1), Affe (1), Schaf (1)
Applikationen Western Blot (WB) (70), Immunfluoreszenz (IF) (60), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (9), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
Konjugate Biotin (4), HRP (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitope C-Term (4), AA 544-776 (1)