HMGN1 Antikörper (pSer20, pSer24)

Produktnummer ABIN303233

Der Kaninchen Polyklonal anti-HMGN1 Antikörper wird verwendet zum Nachweis von HMGN1 in Proben von Human, Maus, Ratte, Schwein, Hund, Rind (Kuh), Affe, Huhn und Frosch. Er wurde validiert für WB, IF, IHC (p) und EIA.

Kurzübersicht für HMGN1 Antikörper (pSer20, pSer24) (ABIN303233)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reaktivität: Human, Maus, Ratte, Schwein, Hund, Rind (Kuh), Affe, Huhn, Frosch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-HMGN1 Antikörper

Bindungsspezifität: AA 19-28, pSer20, pSer24

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24.

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections (20 μg/mL). Immunofluorescence. Western Blot(1/500-1/2000). ELISA (1/5000-1/25000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Details zu HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Andere Bezeichnung: HMGN1

Hintergrund: HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box," that of the HMGN family, the "nucleosomal binding domain," and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."Synonyms: HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1, Non-histone chromosomal protein HMG-14

Gen-ID: 3150

NCBI Accession: NP_004956

UniProt: P05114

Pathways: Chromatin Binding