CD69 (CD69) Antikörper

Antigen: CD69 (CD69)

Synonyme: CLEC2C

Klonalität: Monoklonal (FN50)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Funktionale Studien (Func)

Produktnummer: ABIN302090

Produktbeschreibung

Weitere Bezeichnung: CD69

Immunogen: anti-µ-stimulated human B lymphocytes

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: FN50

Beschreibung: CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliestinducible cell surface molecules acquired during leukocyte activation. This glycoproteinserves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved inlymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage ofHIV infection, and may be also predictive for clinical response to chemoimmunotherapy.

Spezifität: The antibody FN50 recognizes CD69, an lymphocyte early activation marker.HLDA IV, WS Code A 91

Anwendungen

Applikationshinweise: Flow Cytometry: Recommended dilution: 5 µg/ml. Immunohistochemistry (frozen sections). Functional ApplicationWhen stimulating peripheral blood T cells, FN50 antibody together with TPA synergisticallyincreases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheralblood B cells has similar, but weaker effect.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen , Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).