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Antikörper
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anti-Death-Domain Associated Protein (DAXX) Antikörper
Death-Domain Associated Protein (DAXX) Antikörper
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| Synonyme |
DAP6, EAP1, BING2, MGC126245, MGC126246, MGC150289, DAP-3, 4921514D13Rik, im:6905684, LOC100226911 |
| Klonalität |
Monoklonal (DAXX-03) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Immunpräzipitation (IP), Western Blot (WB), Immunzytochemie (ICC)
Western Blot (WB) (64), Immunfluoreszenz (IF) (37), Durchflusszytometrie (FACS) (23), Immunhistochemie (Paraffinschnitte) (IHC (p)) (17), ELISA (16), Enzyme Immunoassay (EIA) (15), Immunzytochemie (ICC) (8), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Immunpräzipitation (IP) (8), Immunhistochemie (IHC) (7), Dot Blot (Dot) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN302079 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
200,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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Daxx
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Immunogen
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His6-tagged protein corresponding to amino acids 580-740 of human Daxx.
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Format
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Purified
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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DAXX-03
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Beschreibung
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Daxx is an apoptosis-modulating death-domain associated protein with functions intranscriptional regulation. Daxx functions bot in cytoplasm, where it interacts with Fas, andin nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependentregulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells toapoptosis, but on the other hand, this protein may also serve in preventing apoptosis in theearly embryo. Even regarding the transcription, Daxx can serve both as a corepressor and acoactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm,where in regulates sodium hydrogen exchanger isoform 1 (NHE1).
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Spezifität
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The antibody DAXX-03 reacts with C-terminal part of Daxx, a 110 kDa intracellular protein.
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Applikationshinweise
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Western Blotting - Recommended dilution: 2 µg/ml
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Apoptose/Nekrose
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Death-Domain Associated Protein (DAXX)", Typ "Antikörper" finden
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Wirte
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Kaninchen (64), Maus (22)
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Reaktivitäten
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Human (84), Maus (38), Ratte (Rattus) (30), Rind (Kuh) (19), Hund (16), Schwein (16), Kaninchen (16), Schaf (15), Affe (7), Pferd (1)
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Applikationen
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Western Blot (WB) (64), Immunfluoreszenz (IF) (37), Durchflusszytometrie (FACS) (23), Immunhistochemie (Paraffinschnitte) (IHC (p)) (17), ELISA (16), Enzyme Immunoassay (EIA) (15), Immunzytochemie (ICC) (8), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Immunpräzipitation (IP) (8), Immunhistochemie (IHC) (7), Dot Blot (Dot) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Fluor 488 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (7), S495 (2), pSer213 (2), pSer495 (2), pSer517 (2), pSer671 (2), AA 272-291 (1), AA 561- 661 (1), S213 (1), S517 (1), pS671 (1)
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