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CD8a Molecule (CD8A) Antikörper (PerCP)

Antigen

CD8a Molecule (CD8A)
Synonyme CD8, MAL, p32, Leu2, Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, MGC136643, zgc:136643, cd8, LOC100136450
Klonalität Monoklonal (MEM-31)
Wirt
Reaktivität
Alternativen

Human
Konjugat
Alternativen PerCP
Applikation
Alternativen Durchflusszytometrie (FACS)
3 Publikationen vorhanden
Produktnummer ABIN302050
Menge 100 tests
Preis 270,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 3 bis 5 Werktagen

Produktbeschreibung
Anwendungen Publikationen Alternativen zurück nach oben

Weitere Bezeichnung CD8
Immunogen Crude thymus membrane fraction.
Isotyp IgG2a
Klon MEM-31
Beschreibung The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/betaheterodimer on majority of MHC I-restricted conventional T cells and thymocytes and asalpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cellsand dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an importantmechanism to control their effector function. Assembly of CD8 alpha-beta but notalpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruitingtriggered TCR complexes to these membrane microdomains as well as affinity of TCR toMHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Spezifität The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cellsurface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cellinteractions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).The antibody does not react with formaldehyde-fixed cells, negative in WesternBlotting application.HLDA III, WS Code T 575

Anwendungen
Publikationen Alternativen zurück nach oben

Applikationshinweise The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Lagerung Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Forschungsgebiet Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, CD Antigene, Oberflächenrezeptoren der Immunzellen
Beschränkungen Nur für Forschungszwecke einsetzbar

Publikationen
Anwendungen Alternativen zurück nach oben

Publikationen Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Estefanuia, Flores, Guomez-Lozano et al.: "Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4402-10, 2007 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Alternativen
Anwendungen Publikationen zurück nach oben

Alternativen zu Antigen "CD8a Molecule (CD8A)", Typ "Antikörper" finden
Wirte Maus (430), Ratte (139), Kaninchen (92)
Reaktivitäten Human (356), Maus (143), Ratte (Rattus) (68), Huhn (33), Katze (27), Affe (23), Schwein (18), Hund (16), Rind (Kuh) (13), Schaf (13), Kaninchen (10), Pferd (8), Meerschweinchen (6), Pute (5), Ziege (4), Vogel (1), Ente (1)
Applikationen Durchflusszytometrie (FACS) (524), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (196), Immunpräzipitation (IP) (121), Immunhistochemie (Paraffinschnitte) (IHC (p)) (66), Immunfluoreszenz (IF) (62), Western Blot (WB) (54), ELISA (33), Immunhistochemie (IHC) (22), Immunzytochemie (ICC) (16), Funktionale Studien (Func) (9), Blockierende Antikörper (Inhibition) (6), Zytotoxizitätstest (CyTox) (6), Enzyme Immunoassay (EIA) (6), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (3), ELISA (Detektionsantikörper) (2), Dot Blot (Dot) (1), Radioimmunoassay (RIA) (1), To be determined by user (TBD) (1)
Konjugate FITC (108), Biotin (67), PE (61), APC (23), Cy5 (12), RPE (12), PE,Cy7 (8), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 700 (2), PE,Texas Red (TR) (2), APC,Cy5.5 (1), Cy5.5,PE (1), Cy7,PE (1), DY647 (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Epitope C-Term (1), N-Term (1)