SRD5A1 Antikörper (AA 2-13)

Produktnummer ABIN297922

Dieser Ziege Polyklonal Antikörper detektiert spezifisch SRD5A1 in IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für SRD5A1 Antikörper (AA 2-13) (ABIN297922)

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SRD5A1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SRD5A1 Antikörper

Bindungsspezifität: AA 2-13

Spezifität: Human SRD5A1.

Homologie: Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla, Marmoset (92%) Ajellomyces (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide ATATGVAEERLLC from the N-terminus of human SRD5A1 (NP_001038.1). Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla, Marmoset (92%), Ajellomyces (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SRD5A1

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Andere Bezeichnung: 5-Alpha Reductase / SRD5A1

Hintergrund: Name/Gene ID: SRD5A1

Synonyms: SRD5A1, 5-alpha reductase, SR type 1, Steroid 5-alpha-reductase 1, S5AR 1

Gen-ID: 6715

NCBI Accession: NP_001038

UniProt: P18405

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process