SEMA3E Antikörper (AA 762-775)

Produktnummer ABIN297629

Der Ziege Polyklonal anti-SEMA3E Antikörper wird verwendet zum Nachweis von SEMA3E in Proben von Human. Er wurde validiert für IHC, ELISA und IHC (p).

Kurzübersicht für SEMA3E Antikörper (AA 762-775) (ABIN297629)

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SEMA3E Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SEMA3E Antikörper

Bindungsspezifität: AA 762-775

Spezifität: Human SEMA3E.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Horse (93%) Hamster, Elephant, Panda, Bat, Bovine, Pig (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KPEHYRLPRHTLDS from the C-terminus of human SEMA3E (NP_036563.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Horse (93%), Hamster, Elephant, Panda, Bat, Bovine, Pig (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SEMA3E

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Andere Bezeichnung: Semaphorin 3E / SEMA3E

Hintergrund: Name/Gene ID: SEMA3E

Synonyms: SEMA3E, M-SEMAH, SEMAH, Semaphorin 3E, M-SemaK, Semaphorin-3E, Coll-5, KIAA0331

Gen-ID: 9723

NCBI Accession: NP_036563

UniProt: O15041