ERG Antikörper (AA 469-481)

Produktnummer ABIN295774

Dieser Ziege Polyklonal Antikörper detektiert spezifisch ERG in IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Affe.

Kurzübersicht für ERG Antikörper (AA 469-481) (ABIN295774)

Target: ERG (ERG, ETS transcription factor (ERG))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ERG Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ERG Antikörper

Bindungsspezifität: AA 469-481

Spezifität: Human ERG. This antibody is expected to recognise both reported isoforms (NP_891548.1, NP_004440.1)

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Platypus, Lizard, Xenopus (92%) Rabbit, Turkey, Chicken (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-PNTRLPTSHMPSH from the C-terminus of human ERG (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Platypus, Lizard, Xenopus (92%), Rabbit, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ERG

Target: ERG (ERG, ETS transcription factor (ERG))

Andere Bezeichnung: ERG

Hintergrund: Name/Gene ID: ERG

Synonyms: ERG, ERG-3, Ets-related, TMPRSS2/ERG fusion, Transcriptional regulator ERG, p55, Transforming protein ERG

Gen-ID: 2078

NCBI Accession: NP_891548, NP_004440, NP_001129626, NP_001129627

UniProt: P11308