MBD3 Antikörper (AA 215-230)

Produktnummer ABIN294235

Dieser Maus Monoklonal Antikörper detektiert spezifisch MBD3 in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Hund, Rind (Kuh), Pferd, Schwein, Fledermaus, Huhn und Xenopus laevis.

Kurzübersicht für MBD3 Antikörper (AA 215-230) (ABIN294235)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reaktivität: Human, Hund, Rind (Kuh), Pferd, Schwein, Fledermaus, Huhn, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MBD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 106B691

Produktdetails anti-MBD3 Antikörper

Bindungsspezifität: AA 215-230

Spezifität: The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100 % ), and human and mouse MBD2 (93 %).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%) Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%) Salmon (88%).

Aufreinigung: Protein G purified

Immunogen: Amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%), Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%), Salmon (88%).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide. Sourced in TCS.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Andere Bezeichnung: MBD3

Hintergrund: Name/Gene ID: MBD3

Synonyms: MBD3

Gen-ID: 53615

UniProt: O95983

Pathways: Chromatin Binding