CCR10 Antikörper (AA 350-362)

Produktnummer ABIN291274

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CCR10 in WB, IHC, FACS und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CCR10 Antikörper (AA 350-362) (ABIN291274)

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCR10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CCR10 Antikörper

Bindungsspezifität: AA 350-362

Spezifität: In A375, a ~50 kD band is observed. Is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2 % formaldehyde and permeabilized with 0.1 % saponin.

Aufreinigung: Protein G purified

Immunogen: A peptide corresponding to amino acids 350-362 of human CCR10. In this region, mouse and human amino acid sequences are 100% identical.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu CCR10

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Andere Bezeichnung: CCR10 / GPR2

Hintergrund: Name/Gene ID: CCR10
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR10, C-C CKR-10, CC-CKR-10, CCR-10, C-C chemokine receptor type 10, CC chemokine receptor 10, G-protein coupled receptor 2, GPR2, G protein-coupled receptor 2

Gen-ID: 2826

UniProt: P46092