Adducin 1 (Alpha) (ADD1) (pSer726) Antikörper

Antigen: Adducin 1 (Alpha) (ADD1)

Synonyme: ADDA, MGC3339, MGC44427, AI256389, MGC124621, ADD1, cb787, bZ1H20.1, MGC158352, zgc:158352, DKFZp459A0431, add1-a, add2, adda, MGC69376

Epitope: pSer726

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN273529

Produktbeschreibung

Weitere Bezeichnung: ADD1 (Phospho-Ser726)

Swiss-Prot: P35611

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S P -F-L).

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Spezifität: ADD1 (Phospho-Ser726) antibody detects endogenous levels of ADD1 only when phosphorylated at serine 726.

Anwendungen

Applikationshinweise: WB: 1:500~1:1000 IHC: 1:50~1:100

Konzentration: 100µg/100µl

Reinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Lagerung: Store at -20° C /1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using ADD1 (Phospho- Ser726) antibody (E011182).

Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 (Ab-726) antibody (E021189, Lane 1 and 2) and ADD1 (Phospho- Ser726) antibody (E011182, Lane 3 and 4).