YBX2 Antikörper

Antigen: YBX2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN272262

Produktbeschreibung

Produktmerkmale: Synonyms: Contrin, DBPC, CSDA3, MSY2, Y-box-binding protein 2, DNA-binding protein C, Germcell-specific Y-box-binding protein, MSY2 homolog

Swiss-Prot: Q9Y2T7

Kreuzreaktivität: Human, Maus

Beschreibung: Contrin (MSY2 in mouse) and YB-2 (MSY3/4 in mouse) belong to the Y-box family ofmultifunctional proteins that regulate both transcription and translation. Y-box proteinsinteract with a wide variety of nucleic acid structures to act as transcription factors andmRNA masking proteins. The modular structure of Y-box proteins includes a highlyconserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shockproteins) and four basic C-terminal domains containing arginine clusters and aromaticresidues. MSY2 (Contrin) is a expressed in testis and ovary, where it may represstranslation of parental mRNA. The gene encoding human Contrin maps to chromosome17p11. 2-13. 1. MSY3/4 (YB-2) is also known as DNA binding protein A and is highlyexpressed in testis, heart and muscle. Contrin and YB-2 bind to the consensus sequence5'-UCCAUCA-3' contained in the Y-box element.

Spezifität: This antibody detects endogenous levels of MSY2 protein. Species Reactivity: Tested: Human and Mouse.

Anwendungen

Applikationshinweise: ELISA: 1/10000~1/20000. Western Blot: 1/500~1/1000. Immunohistochemistry: 1/50~1/200. Immunofluorescence: 1/50~1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Aff - Purified

Buffer: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azideas preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar