NCF4 Antikörper

Produktnummer ABIN272048

Der Kaninchen Polyklonal anti-NCF4 Antikörper wird verwendet zum Nachweis von NCF4 in Proben von Human und Maus. Er wurde validiert für IHC (p).

Kurzübersicht für NCF4 Antikörper (ABIN272048)

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCF4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCF4 Antikörper

Spezifität: This antibody detects endogenous levels of p40 phox protein. (region surrounding Arg149)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Reinheit: > 95 % pure by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE!

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Andere Bezeichnung: NCF-4

Hintergrund: Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase. However, p40-phox appears to downregulate oxidase function as well, by competing with an SH3 domain interaction between other essential oxidase components.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox

Molekulargewicht: approx. 39 kDa

Gen-ID: 4689

NCBI Accession: NP_000622

UniProt: Q15080