S1PR1 Antikörper (C-Term)

Produktnummer ABIN264982

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch S1PR1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 7+ Publikationen erwähnt.

Kurzübersicht für S1PR1 Antikörper (C-Term) (ABIN264982)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S1PR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-S1PR1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody reacts to EDG1.

Aufreinigung: Purified

Immunogen: Synthetic peptide derived from the C-terminal of the EDG-1 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 5-10 μg/mL, Detects an approximately 44 kDa band in RH7777 cellstransfected with full length human EDG-1. Due to low expression of EDG receptors, werecommend use of Pierce Femto Signal substrate for western blot development.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Referenzen für anti-S1PR1 Antikörper (ABIN264982)

Zhang, Loyd, Randall, Waddell, Kriwacki, Ney: "A short linear motif in BNIP3L (NIX) mediates mitochondrial clearance in reticulocytes." in: Autophagy, Vol. 8, Issue 9, pp. 1325-32, (2013) (PubMed).

Goparaju, Jolly, Watterson, Bektas, Alvarez, Sarkar, Mel, Ishii, Chun, Milstien, Spiegel: "The S1P2 receptor negatively regulates platelet-derived growth factor-induced motility and proliferation." in: Molecular and cellular biology, Vol. 25, Issue 10, pp. 4237-49, (2005) (PubMed).

Matsushita, Morrell, Lowenstein: "Sphingosine 1-phosphate activates Weibel-Palade body exocytosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 31, pp. 11483-7, (2004) (PubMed).

Toman, Payne, Watterson, Maceyka, Lee, Milstien, Bigbee, Spiegel: "Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extension." in: The Journal of cell biology, Vol. 166, Issue 3, pp. 381-92, (2004) (PubMed).

Yamaguchi, Kitayama, Takuwa, Arikawa, Inoki, Takehara, Nagawa, Takuwa: "Sphingosine-1-phosphate receptor subtype-specific positive and negative regulation of Rac and haematogenous metastasis of melanoma cells." in: The Biochemical journal, Vol. 374, Issue Pt 3, pp. 715-22, (2003) (PubMed).

Cummings, Parinandi, Zaiman, Wang, Usatyuk, Garcia, Natarajan: "Phospholipase D activation by sphingosine 1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 30227-35, (2002) (PubMed).

Graeler, Goetzl: "Activation-regulated expression and chemotactic function of sphingosine 1-phosphate receptors in mouse splenic T cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 14, pp. 1874-8, (2002) (PubMed).

Details zu S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: EDG-1 / S1PR1

Hintergrund: EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocytecontaining and other tissues.Synonyms: CHEDG1, D1S3362, ECGF1, EDG1, Endothelial differentiation G-protein coupled receptor 1, FLJ58121, S1P receptor 1, S1PR1, Sphingosine 1-phosphate receptor 1

Gen-ID: 1901

NCBI Accession: NP_001391

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2