Human Adenovirus type 2 E1A (HAdV-2 E1A) Antikörper

Produktnummer ABIN264328

Dieser Maus Monoklonal Antikörper detektiert spezifisch Human Adenovirus type 2 E1A in IP und IF. Es zeigt Reaktivität gegenüber Proben von Human Adenovirus 2.

Kurzübersicht für Human Adenovirus type 2 E1A (HAdV-2 E1A) Antikörper (ABIN264328)

Target: Human Adenovirus type 2 E1A (HAdV-2 E1A)

Reaktivität: Human Adenovirus 2

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: M73

Produktdetails

Produktmerkmale: Synonyms: Adenovirus Early Region 1, Early E1A 32 kDa protein

Aufreinigung: Purified

Immunogen: Full length recombinant adenovirus 2 E1A protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation (1-2 μg/mL): Adenovirus 2E1A recognizes adenovirus 2 and 5 E1Aproteins, 3 bands at 30-50 kDa, as well as, E1A associated proteins. Immunofluorescence: 2-5 μg/mL.293 cells can be used as positive control and HS27 cells as negative control. Positive Control: 293 cells are human embryonic kidney cells that have been transformedwith the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: Human Adenovirus type 2 E1A (HAdV-2 E1A)

Andere Bezeichnung: Adenovirus Type 2 E1A

Substanzklasse: Viral Protein

Hintergrund: The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11 % of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary Cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.Synonyms: Adenovirus Early Region 1, Early E1A 32 kDa protein

UniProt: P03254