Fucose Antikörper

Antigen: Fucose

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Höhere Pflanzen

Applikation: Western Blot (WB)

Produktnummer: ABIN249420

Produktbeschreibung

Immunogen: core fucose residues bound to the N-glycan in alpha 1,3

Format: Lyophilized

Beschreibung: This antibody specifically cross-reacts against fucose residues bound to theprotein N-glycans in alpha 1,3. This residue is characterisitc of the plant proteinN-glycans and is absent in protein N-glycans from animals. This residue is addedin the Golgi apparatus.

Spezifität: Predicted reactivity: higher plants.

Anwendungen

Applikationshinweise: Recommended Dilution 1 ug/10 ml of incubation buffer, with standard ECL (WB). Additional Information: Alpha (1,3) fucose is present not only in plants but also in some invertebrates(such as nematodes, bees, etc.) . However, cross-reaction with glycoproteins fromthese organisms is weaker than the one observed in plants. This sugar residuedoes not exist in mammals, in their endogenous glycoproteins.

Buffer: Affinity purified serum in PBS pH 7.4

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

From left to right: total cell extract from Arabidopsis thaliana wild type and cell extracts from different mutants defective in fucosyltransferases (data not published yet)