Secretion-Associated and Ras-Related Protein 1 (Sar1) Antikörper

Antigen: Secretion-Associated and Ras-Related Protein 1 (Sar1)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Wheat (Triticum), Proso millet (Panicum miliaceum), Guinea gras (Panicum maximum), Barnyard Grass (Echinochloa crus-galli), Lovegrass (Eragrostis neomexicana), Large Crabgrass (Digitaria sanguinalis), Farne

Applikation: Western Blot (WB), Immunolokalisation (IL)

Produktnummer: ABIN249384

Produktbeschreibung

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7

Format: Lyophilized

Beschreibung: Sar1 belongs to a small GTPase superfamily and GTP binging activity. Thisprotein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER andthe Golgi. Coat protein complexes involving membrane-associated GTP bingingproteins – Arf1 and Sar1p for COPI and COPII are needed for formation ofCOP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited ontothe membranes of the ER.

Spezifität: Predicted reactivity: dicots including Brassica campestris, Solanum tuberosum monocots includingTriticum aestivum, Oryza sativa, Zea mays, tree: Populus trichocarpa, mossPhyscomitrella patens. Not reactive in: Nicotiana tabacum.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 500 with standard ECL (WB), 1: 50 (IG). Additional Information: For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES.LR White resin has been used. Tested species were: Triticum aestivum, Panicummiliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana,Digitaria sanguinalis. Publication in preparation.

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Pimpl, Movafeghi, Coughlan et al.: "In situ localization and in vitro induction of plant COPI-coated vesicles." in: The Plant cell, Vol. 12, Issue 11, pp. 2219-36, 2001 (PubMed).

Tnani, La: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, 2011 (PubMed).