Glutamine Oxoglutarate Aminotransferase (GOGAT) Antikörper

Antigen: Glutamine Oxoglutarate Aminotransferase (GOGAT)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Miscanthus giganteus, Phaseolus vulgaris, Cordgrass (Spartina), Maize/Corn (Zea mays)

Applikation: Western Blot (WB)

Produktnummer: ABIN249351

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide well conserved in known GOGAT sequencesfrom different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7-2 andFd-GOGAT 2 Q9T0P4

Format: Lyophilized

Beschreibung: Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzymeinvolved in synthesis of glutamate from glutamine and alpha-ketoglutarate.GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) andNADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants isthe Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 foundon chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms)is highly conserved among plants, red algae, and cyanobacteria.

Spezifität: Predicted reactivity: Medicago trucutula, Ostreococcus lucimarinus, Leptolyngbya boryana, Porphyrapurpurea, Gracilaria tenustipitata, cyanobacteria.

Anwendungen

Applikationshinweise: Recommended Dilution 1 : 1000 with standard ECL (WB) MW 177 | 170-180 kDa depending upon the species.

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

From left to right: Arabidopsis thaliana (1), Phaseaolus vulgaris (2), Zea mays (3), Spartina alterniflora (4), Spartina patens (6). (20 ug of total cellular protein was loaded per lane) A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies.