Membrane-Associated Ring Finger (C3HC4) 6 (MARCH6) (AA 701-712) Antikörper

Antigen: Membrane-Associated Ring Finger (C3HC4) 6 (MARCH6)

Synonyme: TEB4, RNF176, KIAA0597, MARCH-VI, mKIAA0597, 3830408G03, F830029L24Rik, RGD1310927, RGD1565757, MARCH6, im:7161807, wu:fa98a02, si:dkey-40c17.2, DKFZp468P115

Epitope: AA 701-712

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN238654

Produktbeschreibung

Weitere Bezeichnung: TEB4 / MARCH-VI (aa 701 to 712)

Gen-ID: 10299, 223455 (mouse)

Immunogen: C-QGRRVIFQKVKE

Format: Affinity-purified

Beschreibung: All names and symbols: MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, MARCH-VI, RNF176, TEB4, membrane-associated RING-CH protein VI. Official Symbol: MARCH6. RefSeq number(s): NP_005876.2

Spezifität: Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 100kDa band observed in Human Lung lysates (calculated MW of 103kDa according to NP_005876.2). Recommended concentration: 0.2-0.5μg/ml. An additional band of unknown identity was also consistently observed at 18kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinheit: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN238654 (0.2 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Kreft, Wang, Hochstrasser: "Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 4646-53, 2006 (PubMed).