beta-1,3-Galactosyl-O-Glycosyl-Glycoprotein beta-1,6-N-Acetylglucosaminyltransferase 3 (GCNT3) (AA 273-284) Antikörper

Antigen: beta-1,3-Galactosyl-O-Glycosyl-Glycoprotein beta-1,6-N-Acetylglucosaminyltransferase 3 (GCNT3)

Synonyme: GNTM, C24GNT, C2GNT2, C2GNTM, C2/4GnT

Epitope: AA 273-284

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN238637

Produktbeschreibung

Weitere Bezeichnung: GCNT3 (aa 273 to 284)

Gen-ID: 9245

Immunogen: C-RDTLHLTNKKKD

Format: Affinity-purified

Beschreibung: All names and symbols: GCNT3, glucosaminyl (N-acetyl) transferase 3, mucin type, C2/4GnT, C2GnT-M, C2GnT2, GnT-M, beta1,6-N-acetylglucosaminyltransferase. Official Symbol: GCNT3. RefSeq number(s): NP_004742.1

Spezifität: Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx. 50kDa band observed in Human Lymph Node lysates (calculated MW of 50.9kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN238637 (0.3µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing GCNT3 (RC202007) and probed with ABIN238637 (mock transfection in first lane), tested by Origene.

Publikationen

Publikationen: Schwientek, Yeh, Levery et al.: "Control of O-glycan branch formation. Molecular cloning and characterization of a novel thymus-associated core 2 beta1, 6-n-acetylglucosaminyltransferase." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11106-13, 2000 (PubMed).