Naked Cuticle Homolog 2 (Drosophila) (NKD2) Antikörper

Antigen: Naked Cuticle Homolog 2 (Drosophila) (NKD2)

Synonyme: AW212591, 2210403L10Rik, Naked2, nkd2b

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN238609

Produktbeschreibung

Weitere Bezeichnung: NKD2

Gen-ID: 85409, 72293 (mouse), 308068 (rat)

Immunogen: C-HKRYRQKGREGHS

Format: Affinity-purified

Beschreibung: All names and symbols: NKD2, naked cuticle homolog 2 (Drosophila), Dvl-binding protein NKD2, naked cuticle homolog 2, naked cuticle-2. Official Symbol: NKD2. RefSeq number(s): NP_149111.1

Spezifität: Human, Mouse, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 80kDa, 38kDa and 26kDa in Mouse Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.5kDa according to Mouse NP_082462.2 and of 50.1kDa according to Human NP_149111.1. All the detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Stammzellen, Wnt

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Zhang, Cagatay, Amanai et al.: "Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4454-64, 2007 (PubMed).