Arylsulfatase B (ARSB) Antikörper

Antigen: Arylsulfatase B (ARSB)

Synonyme: ASB, G4S, MPS6, As1, As-1, As-1r, As-1s, As-1t, As1-r, As1-s, As1-t, Asr-1, Ast-1, AI480648, 1110007C02Rik, ARSB

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN238566

Produktbeschreibung

Weitere Bezeichnung: Arylsulfatase B

Gen-ID: 411

Immunogen: C-KLARGHTNGTKPLD

Format: Affinity-purified

Beschreibung: All names and symbols: ARSB, arylsulfatase B, ASB, G4S, MPS6, N-acetylgalactosamine-4-sulfatase. Official Symbol: ARSB. RefSeq number(s): NP_000037.2, NP_942002.1

Spezifität: This antibody is expected to recognise both reported isoforms (NP_000037.2, NP_942002.1). Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 45kDa band observed in Human Heart lysates (calculated MW of 46.0kDa according to NP_942002.1). Recommended concentration: 0.03-0.1µg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres. Recommended concentration, 5-10µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN238566 (0.03µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN238566 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publikationen

Publikationen: Bhattacharyya, Look, Tobacman: "Increased arylsulfatase B activity in cystic fibrosis cells following correction of CFTR." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 380, Issue 1-2, pp. 122-7, 2007 (PubMed).