Chromosome 14 Open Reading Frame 169 (C14orf169) Antikörper

Antigen: Chromosome 14 Open Reading Frame 169 (C14orf169)

Synonyme: NO66, MAPJD, FLJ21802, LOC453015

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN238538

Produktbeschreibung

Weitere Bezeichnung: C14orf169 / NO66

Gen-ID: 79697, 71952 (mouse)

Immunogen: C-GAQHSDSKDPR

Format: Affinity-purified

Beschreibung: All names and symbols: C14orf169, NO66, chromosome 14 open reading frame 169, FLJ21802, MAPJD. Official Symbol: C14orf169. RefSeq number(s): NP_078920.2

Spezifität: Human, Mouse, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in lysates of cell line A431 (calculated MW of 71.1kDa according to NP_078920.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN238538 (0.3µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Eilbracht, Reichenzeller, Hergt et al.: "NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin." in: Molecular biology of the cell, Vol. 15, Issue 4, pp. 1816-32, 2004 (PubMed).