CD4 Molecule (CD4) Antikörper

Antigen: CD4 Molecule (CD4)

Synonyme: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Klonalität: Monoklonal (MEM-16)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN238451

Produktbeschreibung

Produktmerkmale: Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Weitere Bezeichnung: CD4

Swiss-Prot: P01730

Immunogen: HPB cell line (human peripheral blood leukemia T-cells).

Kreuzreaktivität: Human

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: MEM-16

Beschreibung: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulinsupergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-likeV-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consistsof 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain ofCD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1),HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds toCD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1),L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHCclass II molecules) and HIV infection (human immunodeficiency virus, CD4 is primaryreceptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-celladhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigenpresentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost completeabsence in patients blood, tissue and organs (SCID immunodeficiency).

Spezifität: The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55 kDatransmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) andalso on monocytes, tissue macrophages and granulocytes. Negative Species: Porcine.

Anwendungen

Applikationshinweise: Flow Cytometry: 10 μg/mlPositive control: Peripheral blood lymphocytesImmunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar