Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) Antikörper (Biotin)

Antigen: Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1)

Synonyme: B1, S7, Bp35, CD20, MS4A2, LEU-16, MGC3969, Cd20, Ly-44, Ms4a2, AA960661, FcRB, Fce1b, Ms4a1, Fcer1b, Fcrbeta, FCIGA, MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like

Klonalität: Monoklonal (LT20)

Wirt: Maus

Reaktivität: Human

Konjugat: Biotin

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN238432

Produktbeschreibung

Weitere Bezeichnung: CD20

Swiss-Prot: P11836

Immunogen: Normal human lymphocytes from lymph node

Kreuzreaktivität: Human

Isotyp: IgG2a

Klon: LT20

Beschreibung: CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non- glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.

Spezifität: The antibody LT20 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Anwendungen

Applikationshinweise: This Biotinylated antibody is suitable for indirect Immunofluorescence analysis by Flow Cytometryn (1/150). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Krebs, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar