CD163 Molecule (CD163) Antikörper

Antigen: CD163 Molecule (CD163)

Synonyme: CD163, DMBT1, M130, MM130, CD163v2, CD163v3

Klonalität: Monoklonal (5C6-FAT)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN238373

Produktbeschreibung

Produktmerkmale: Synonyms: M130, Hemoglobin scavenger receptor, Macrophage marker, Scavenger receptorcysteine-rich type 1 protein M130

Weitere Bezeichnung: CD163

Swiss-Prot: Q86VB7

Immunogen: Human CD163. The epitope has not been further characterized.

Kreuzreaktivität: Human

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 5C6-FAT

Beschreibung: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and isupregulated by glucocorticoids and IL-10. The extracellular portion of the receptor isregularly shed and can be found in the circulation. An important function of CD163 seemsto be in the adhesion of monocytes to activated endothelial cells. CD163-positive cellsinclude skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and somethymus macrophages. The antigen is also found abundantly in human term placenta, andregularly in acute and chronic inflammatory lesions.

Spezifität: Monoclonal antibody 5C6 FAT recognizes a membrane glycoprotein on monocytes andmacrophages which is expressed in intermediate and late inflammatory stages. Antigen distribution: Isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days inculture. Does not react with lymphocytes, granulocytes or platelets. Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffercells, few alveolar macrophages, a major population of macrophages in the placenta,varying degrees of macrophages in inflamed tissues, including tumorous tissue depending

Anwendungen

Applikationshinweise: ELISA. Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/4000)Immunohistochemistry on Paraffin Sections: 1 μg/ml (1/2000) (Proteinase K pretreatmentfor antigen retrieval is recommended). Suggested Positive Control: Human placenta. Has been described to work in FACS and Western blots. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 2.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.2

Lagerung: Store original vial at -70°C. Aliquots of stock solution: at -70°C. Do not freeze working dilutions. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Kardiovaskuläres System, Artherosklerose, CD Antigene, Angeborene Immunität, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar