CD4 Molecule (CD4) Antikörper

Antigen: CD4 Molecule (CD4)

Synonyme: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Klonalität: Monoklonal (13B8-2)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Funktionale Studien (Func), Western Blot (WB)

Produktnummer: ABIN238364

Produktbeschreibung

Produktmerkmale: Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Weitere Bezeichnung: CD4

Swiss-Prot: P01730

Immunogen: Human thymocytes

Kreuzreaktivität: Human

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 13B8-2

Beschreibung: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to theimmunoglobulin superfamily. CD4 is present on a subset of T lymphocytes(helper/inducer T cells) and is also expressed at a lower level on monocytes, tissuemacrophages and granulocytes. The antigen is involved in binding to MHC class IImolecules. The intracellular domain of the antigen is associated with p56lck proteintyrosine kinase.

Spezifität: The CD4 antigen is a monomeric transmembrane glycoprotein with a molecular weight (Mr)of 60 kDa. CD4 molecule is expressed on a specific subset of peripheral blood T lymphocytes named''helper/inducer''. The CD4 antige is present on approximately 45% of peripheral bloodlymphocytes (T4 lymphocytes), 80% of thymocytes and with a lower density of 100% ofmonocytes and on some neutrophils. Add. Information: CD4 acts as an accessory molecule to the T cell receptor (TcR) complex during T-cellactivation restricted to the Class II major Hitocompatibility Complex (HHC). The CD4 antigen is also known to be one of the human Immunodeficiency Virus (HIV)receptors. The T lymphocyte subset that expresses CD4 is involved in T-T, T-B, and B-MacrophageCellular Interactions.

Anwendungen

Applikationshinweise: Flow cytometry: Studies of CD4 expressing cells. Fluorescence microscopy or Flow cytometry: 2 g/5x10e5 cells/test. Immunohistochemistry: Working dilution 1/25-1/50. Only suitable on cryostat sections or cell smears. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.2 mg/ml (prior to lyophilization)

Reinigung: Azide Free

Buffer: PBS containing 1 mg/ml BSA as stabilizer without preservatives.Reconstitution: Reconstitute with 1 ml distilled water.

Lagerung: Store lyophilized (preferably in a desiccator) at 2-8°C and reconstituted (in aliquots) at-20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar