Acid Phosphatase, Prostate (ACPP) Antikörper

Antigen: Acid Phosphatase, Prostate (ACPP)

Synonyme: PAP, ACP3, ACP-3, Lap, Ppal, AI324033, A030005E02Rik, pap, Acpp11, RNACPP11, ACPP, MGC159906, acpt, MGC130636

Klonalität: Monoklonal (LT-3D1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Zell-ELISA (cELISA)

Produktnummer: ABIN238332

Produktbeschreibung

Weitere Bezeichnung: Prostatic Acid Phosphatase (PAP)

Swiss-Prot: P15309

Immunogen: genetic immunisation with cDNA encoding human PAP (Sequence: AAH07460)

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: LT-3D1

Beschreibung: Human prostatic acid phosphatase (PAP) is a non-specific phosphomonoesterase, synthesised and secreted into seminal plasma under androgenic control. Human PAP is a 100 kDa glycoprotein containing two subunits of approximately 50 kDa each. It catalyses the dephosphorylation of organic monophosphate esters, demonstrating optimum activity at an acid pH. Produced by the prostatic epithelium, serum levels of PAP are very low in healthy individuals, but are often elevated in malignant and benign prostatic disease while it has been used as a marker of diagnosis and therapy control of cancer of the prostate gland.

Anwendungen

Applikationshinweise: Flow cytometry: 1.2 μg/10^6 cells. CELISA: 1:200 - 1:400

Konzentration: 2mg/ml

Reinigung: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page

Buffer: phosphate buffered saline, pH 7.2

Forschungsgebiet: Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

FACS analysis of BOSC23 cells using LT-3D1. BOSC23 cells were transiently transfected with an expres-sion vector encoding either PAP (red curve) or an irrelevantprotein (control transfectant: black curve). Binding of LT-3D1wasdetected with a PE-conjugated secondary antibody. A positivesignal was obtained only with PAP transfected cells.

SDS-PAGE analysis of purified LT-3D1 monoclonalantibody. Lane 1: molecular weight marker, Lane 2: 2 μg ofpurified LT-3D1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.