Filamentous Phages M13 Fd F1 Antikörper (Biotin)

Antigen: Filamentous Phages M13 Fd F1

Klonalität: Monoklonal (B62-FE2)

Wirt: Maus

Konjugat: Biotin

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN235800

Produktbeschreibung

Weitere Bezeichnung: Filamentous Phages, M13, fd, F1

Immunogen: fd phages from E. coli F+ strain (JM109)

Format: Liquid

Isotyp: IgG2b

Klon: B62-FE2

Beschreibung: MAb to Filamentous Phages M13/fd/F1 Monoclonal Antibody to Filamentous Phages, M13, fd, F1 Biotin conjugated

Spezifität: Binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.

Anwendungen

Applikationshinweise: Phage Display (immunoassays for the identification of recombinant antigen or antibody phages) Immunoblotting ELISA (1:5,000?1:10,000) Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Konzentration: 100ug/ml

Reinigung: Protein A chromatography. Source: Tissue culture

Buffer: PBS, pH 7.4 containing 0.5% BSA. Preservative: 0.09% Sodium azide

Lagerung: Store at 2-8°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Micheel, Fiebach, Karsten et al.: "Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP)." in: European journal of cancer & clinical oncology, Vol. 19, Issue 9, pp. 1239-46, 1983 (PubMed).

Kneissel, Queitsch, Petersen et al.: "Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae)." in: Journal of molecular biology, Vol. 288, Issue 1, pp. 21-8, 1999 (PubMed).

Rondot, Koch, Breitling et al.: "A helper phage to improve single-chain antibody presentation in phage display." in: Nature biotechnology, Vol. 19, Issue 1, pp. 75-8, 2001 (PubMed).