Teratocarcinoma-Derived Growth Factor 1 (TDGF1) Antikörper

Antigen: Teratocarcinoma-Derived Growth Factor 1 (TDGF1)

Synonyme: CR, CRGF, CRIPTO, Cripto-1, CR1, cripto, cb85, tdgf1, wu:fe01d04, zgc:109829, cr1, cr1a, frl1, xcr1, frl-1, TDGF1, tdgf3, cripto-3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN233828

Produktbeschreibung

Weitere Bezeichnung: Cripto-1 (TDGF1)

Gen-ID: NP_003203

Swiss-Prot: P13385

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.

Format: Liquid (sterile filtered)

Beschreibung: Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGF beta ) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.

Spezifität: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human Cripto-1 protein. A BLAST analysis was used to suggest limited cross-reactivity with Cripto-1 from mouse based on a 70% homology with the immunizing sequence. Expect cross-reactivity with human Cripto-3 (TDGF2) based on very high levels of sequence conservation. Cross-reactivity with Cripto-1 from other sources has not been determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:105,000 WESTERN BLOT 1:1000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to Cripto-1 by western blotting in the appropriate cell lysate or extract. Cripto-1 is reported as a 188 amino acid protein with a molecular weight of 21.1 kDa.

Konzentration: 0.75 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) antibody

Publikationen

Publikationen: Malchenko, Galat, Seftor et al.: "Cancer hallmarks in induced pluripotent cells: New insights." in: Journal of cellular physiology, 2010 (PubMed).