SLC6A3 Antikörper (N-Term)
Kurzübersicht für SLC6A3 Antikörper (N-Term) (ABIN215796)
Target
Alle SLC6A3 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- N-Term
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Spezifität
- Recognizes Dopamine transporter (DAT). Species cross-reactivity: Rat and mouse. Weakly reacts with human and canine.
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.
Type of Immunogen: Synthetic peptide -
Isotyp
- IgG
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Applikationshinweise
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Approved: ELISA (0.5 - 1 μg/mL), IHC, IHC-Fr, IP, WB (1 - 10 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and ELISA. Western Blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD. Immunoprecipitation: 2-10 μg. Immunohistochemistry: 2-10 μg/mL on frozen sections of tissues fixed with 4 % paraformaldehyde. ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide per well. -
Kommentare
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Target Species of Antibody: Rat
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS, pH 7.4, 0.1 % BSA, 0.05 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))
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Andere Bezeichnung
- SLC6A3 / Dopamine Transporter
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Hintergrund
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Name/Gene ID: SLC6A3
Subfamily: Neurotransmitter:sodium symporter
Family: Transporter
Synonyms: SLC6A3, DA transporter, DAT1, DAT, Dopamine transporter -
Gen-ID
- 6531
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UniProt
- Q01959
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Pathways
- Dopaminergic Neurogenesis
Target
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