Alpha, beta-Tubulin Dimer Antikörper

Antigen: Alpha, beta-Tubulin Dimer

Klonalität: Monoklonal (TU-08)

Wirt: Maus

Reaktivität: Human, Schwein, Maus

Applikation: Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN192341

Produktbeschreibung

Immunogen: Microtubule proteins from porcine brain.

Format: Purified

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: TU-08

Beschreibung: The microtubules are intracellular dynamic polymers made up of evolutionarily conservedpolymorphic alpha/beta-tubulin heterodimers and a large number ofmicrotubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments andhave an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plusends and less dynamic minus ends. Microtubules are required for vital processes ineukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellulartransport. Microtubules are also necessary for movement of cells by means of flagella andcilia. In mammalian tissue culture cells microtubules have their minus ends anchored inmicrotubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is anessential for tubulin heterodimer to associate with other heterodimers to form microtubule. Invivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible anda populations of microtubules in cells are on their minus ends either growing or shorteningthis phenomenon is called dynamic instability of microtubules. On a practical level,microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP(eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizesmicrotubules at room temperature for many hours. Using limited proteolysis by enzymesboth tubulin subunits can be divided into N-terminal and C-terminal structural domains.The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that existsin cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. Indifferent species it is coded by multiple tubulin genes that form tubulin classes (in human 6genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypesare expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypesand their posttranslational modifications are responsible for multiple tubulin charge variants -tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structuraldomain.The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulinin tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationallymodified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

Spezifität: The antibody TU-08 recognizes alpha, beta-tubulin heterodimer (porcine brain), a basicintracellular structural unit of microtubules. Alpha- and beta- tubulins form approximately 100kDa tubulin heterodimer, a globular protein that polymerizes to form microtubules.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 5 µg/ml. Positive control: porcine brain lysate. Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.

Konzentration: 1 mg/ml

Reinheit: > 95 %

Reinigung: Purified from ascites by precipitation methods and ion exchange chromatography.

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar