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Antikörper
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anti-Intercellular Adhesion Molecule 1 (ICAM1) Antikörper (APC)
Intercellular Adhesion Molecule 1 (ICAM1) Antikörper (APC)
| Antigen |
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| Synonyme |
BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM |
| Klonalität |
Monoklonal (1H4) |
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Wirt
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Reaktivität
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Human (418), Maus (193), Ratte (Rattus) (116), Rind (Kuh) (60), Schwein (45), Kaninchen (43), Hund (39), Schaf (19), Pferd (16), Huhn (4), Meerschweinchen (2), Hamster (2), Affe (2), Fisch (1)
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Konjugat
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Biotin (60), FITC (54), PE (50), Alexa Fluor 488 (14), Alexa Fluor 647 (11), PE,Cy5 (9), APC (7), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Cy3 (7), Cy5 (7), Cy5.5 (7), Cy7 (7), Gold (7), HRP (7), PE,Cy3 (7), PE,Cy5.5 (7), PE,Cy7 (7), RBITC (7), RPE (7), Alexa Flour 488 (6), Pacific Blue (3), Alexa Fluor 700 (2), Alexa Fluor 546 (1), PerCP (1)
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Applikation
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Durchflusszytometrie (FACS) (465), Immunfluoreszenz (IF) (160), Western Blot (WB) (155), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (129), Immunpräzipitation (IP) (115), Immunhistochemie (Paraffinschnitte) (IHC (p)) (85), ELISA (63), Funktionale Studien (Func) (33), Immunhistochemie (IHC) (28), Enzyme Immunoassay (EIA) (24), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (13), Immunzytochemie (ICC) (11), Blocking Antibody (Inhibition) (10), Blockierende Antikörper (Inhibition) (10), Immunoelectron Microscopy (IEM) (7), Immunohistochemistry (Fixed) (IHC (fx)) (5), Intrazelluläre Durchflusszytometrie (ICFC) (4), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2)
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| Produktnummer |
ABIN192333 |
| Menge |
100 tests |
| Preis |
270,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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CD54
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Immunogen
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Raji cells and spleen cells fused with NS1 cells
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Isotyp
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IgG2b
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Klon
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1H4
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Beschreibung
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CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is atransmembrane molecule with 7 potential N-glycosylated sites, expressed on restingmonocytes and endothelial cells and can be upregulated on many other cells, e.g. withlymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also onkeratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion bybinding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction ofCD54 with LFA-1 enhances antigen-specific T-cell activation.
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Spezifität
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The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembraneglycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, Tlymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells,the expression of CD54 is upregulated by activation.
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Applikationshinweise
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The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
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Lagerung
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Forschungsgebiet
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CD Antigene, Oberflächenrezeptoren der Immunzellen , Immunologie, Inflammation, Infektionen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Intercellular Adhesion Molecule 1 (ICAM1)", Typ "Antikörper" finden
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Wirte
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Maus (283), Kaninchen (194), Ratte (99), Hamster (8), Schaf (1)
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Reaktivitäten
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Human (418), Maus (193), Ratte (Rattus) (116), Rind (Kuh) (60), Schwein (45), Kaninchen (43), Hund (39), Schaf (19), Pferd (16), Huhn (4), Meerschweinchen (2), Hamster (2), Affe (2), Fisch (1)
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Applikationen
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Durchflusszytometrie (FACS) (465), Immunfluoreszenz (IF) (160), Western Blot (WB) (155), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (129), Immunpräzipitation (IP) (115), Immunhistochemie (Paraffinschnitte) (IHC (p)) (85), ELISA (63), Funktionale Studien (Func) (33), Immunhistochemie (IHC) (28), Enzyme Immunoassay (EIA) (24), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (13), Immunzytochemie (ICC) (11), Blocking Antibody (Inhibition) (10), Blockierende Antikörper (Inhibition) (10), Immunoelectron Microscopy (IEM) (7), Immunohistochemistry (Fixed) (IHC (fx)) (5), Intrazelluläre Durchflusszytometrie (ICFC) (4), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2)
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Konjugate
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Biotin (60), FITC (54), PE (50), Alexa Fluor 488 (14), Alexa Fluor 647 (11), PE,Cy5 (9), APC (7), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Cy3 (7), Cy5 (7), Cy5.5 (7), Cy7 (7), Gold (7), HRP (7), PE,Cy3 (7), PE,Cy5.5 (7), PE,Cy7 (7), RBITC (7), RPE (7), Alexa Flour 488 (6), Pacific Blue (3), Alexa Fluor 700 (2), Alexa Fluor 546 (1), PerCP (1)
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Epitope
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pTyr512 (9), C-Term (3), AA 253-267 (1), Domain D1 (1)
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Alternativen 
