Vimentin (VIM) Antikörper (PE)

Antigen: Vimentin (VIM)

Synonyme: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Klonalität: Monoklonal (VI-RE-1)

Wirt: Maus

Reaktivität: Human

Konjugat: PE

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN192298

Produktbeschreibung

Weitere Bezeichnung: Vimentin

Immunogen: Bacterially expressed full-length human vimentin

Isotyp: IgG1

Klon: VI-RE-1

Beschreibung: Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to beexpressed during cell differentiation. All primitive cell types express vimentin but in mostnon-mesenchymal cells it is replaced by other intermediate filament proteins duringdifferentiation. Vimentin is expressed in a wide variety of mesenchymal cell types -fibroblasts, endothelial cells etc., and in a number of other cell types derived frommesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth musclecellsand striated muscle, vimentin is often replaced by desmin, however, duringregeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system(lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts.Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule),endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary andsweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells,vimentin is coexpressed with cytokeratin .Furthermore, vimentin is detected in many cells from the neural crest. Particularlymelanocytes express abundant vimentin. In glial cells vimentin is coexpressed with glialfilament acidic protein (GFAP).Vimentin is present in many different neoplasms but is particulary expressed in thoseoriginated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibroushistiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas,malignant melanoma and schwannoma, are virtually always vimentin positive. Mesodermderived carcinomas like renal cell carcinoma, adrenal cortical carcinoma andadenocarcinomas from endometrium and ovary usually express vimentin. Also thyroidcarcinomas are vimentin positive. Any low differentiated carcinoma may express somevimentin.Vimentin is frequently included in the so-called primary panel (together with CD45,cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpressionof other intermediate filament proteins is strongly suggestive of a mesenchymal tumour ormalignant melanoma.

Spezifität: The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament proteinexpressed on a wide variety of mesenchymal and mesodermal cell types. This antibody does not react with: Porcine, Mouse .

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis. Suggested working concentration is 10 µg/ml. Indicated concentration is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Konzentration: 0.1 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with R-Phycoerythrin (PE)

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: Abstammungsmarker, Zytoskelett, Neuronale Stammzellmarker, Zell-/Gewebemarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Intracellular Flow Cytometry analysis