Insulin-Like Growth Factor 2 Receptor (IGF2R) Antikörper (FITC)

Antigen: Insulin-Like Growth Factor 2 Receptor (IGF2R)

Synonyme: MPR1, MPRI, CD222, CIMPR, M6P-R, CI-MPR, Mpr300, AI661837, M6P/IGF2R, mpri, cd222, cimpr, m6p-r, IGF2R

Klonalität: Monoklonal (MEM-238)

Wirt: Maus

Reaktivität: Human, Primaten

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN192247

Produktbeschreibung

Weitere Bezeichnung: CD222

Immunogen: Recombinant Vaccinia virus encoding CD222.

Isotyp: IgG1

Klon: MEM-238

Beschreibung: CD222 (CIMPR, cation-independent mannose 6-phosphate receptor, IGF2 receptor) is aubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 ispresent on the cell surface where it serves as a multifunctional receptor. Intracellular (major)fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modifiedby mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 bindsand internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cellgrowth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor),plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor forheparanase and even for Listeria.

Spezifität: The antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein.The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% inthe plasma membrane and the truncated (220 kDa) form of CD222 is present in human andbovine serum.

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar