PIWIL1 Antikörper (Internal Region)

Produktnummer ABIN190916

Produktdetails anti-PIWIL1 Antikörper

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PIWIL1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: PIWI / HIWI

Sequenz: SNRKDKYDAI KKY

Spezifität: This antibody is expected to recognise both reported isoforms (NP_004755.2, NP_001177900.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2, NP_001177900.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PIWIL1

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Andere Bezeichnung: PIWIL1 (PIWIL1 Produkte)

Synonyme: ziwi antikoerper, PIWI antikoerper, HIWI antikoerper, MIWI antikoerper, piwi like RNA-mediated gene silencing 1 antikoerper, PIWI-like protein 1 antikoerper, putative PIWI-like protein 1 antikoerper, putative argonaut-like protein antikoerper, piwi-like RNA-mediated gene silencing 1 antikoerper, PIWIL1 antikoerper, Tb10.70.5520 antikoerper, LINJ_21_0470 antikoerper, LBRM_21_0470 antikoerper, piwil1 antikoerper, Piwil1 antikoerper

Hintergrund: PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi, MIWI, piwi homolog, piwi-like 1

Gen-ID: 9271, 57749

NCBI Accession: NP_004755, NP_001177900