Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) Antikörper

Antigen: Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H)

Synonyme: CDC68, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68, CG1828, DmelCG1828, DRE4/dSPT16, dspt16, dSpt16, dSPT16, l(3)62Bg, l(3)dre4, spt16, Spt16, Cdc68, Fact140, gtc102, global transcription factor C, SPT16, T12H20.3, T12H20_3, SSF1

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN190913

Produktbeschreibung

Weitere Bezeichnung: FACT / SUPT16H

Gen-ID: 11198

Immunogen: C-DLTNKEGKKPEEK

Format: Affinity-purified

Beschreibung: All names and symbols: SUPT16H, FACT, suppressor of Ty 16 homolog (S. cerevisiae), HGNC:11465, CDC68, FACTP140, FLJ10857, FLJ14010, SPT16/CDC68, chromatin-specific transcription elongation factor large subunit. Official Symbol: SUPT16H. RefSeq number(s): NP_009123.1

Spezifität: Human

Synonyme: CDC68, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68, CG1828, DmelCG1828, DRE4/dSPT16, dspt16, dSpt16, dSPT16, l(3)62Bg, l(3)dre4, spt16, Spt16, Cdc68, Fact140, gtc102, global transcription factor C, SPT16, T12H20.3, T12H20_3, SSF1

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN190913 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Belotserkovskaya, Oh, Bondarenko et al.: "FACT facilitates transcription-dependent nucleosome alteration." in: Science (New York, N.Y.), Vol. 301, Issue 5636, pp. 1090-3, 2003 (PubMed).