Neuroligin 2 (NLGN2) Antikörper

Antigen: Neuroligin 2 (NLGN2)

Synonyme: KIAA1366, NL2, MGC67386, RP23-422L16.24, Nlgn2, GB10066, NLGN2, LOC574368, CCE-L2, NV50031, Nv123771

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN190848

Produktbeschreibung

Weitere Bezeichnung: Neuroligin 2

Gen-ID: 57555, 216856 (mouse), 117096 (rat)

Immunogen: C-NPPDTDIRDPGKK

Format: Affinity-purified

Beschreibung: All names and symbols: neuroligin 2 , KIAA1366, NLGN2. Official Symbol: NLGN2. RefSeq number(s): NP_065846.1

Spezifität: Human, Mouse, Rat, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 90kDa band observed in Human Pancreas lysates (calculated MW of 90.8kDa according to NP_065846.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Neurologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN190848 (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Ichtchenko, Nguyen, Suedhof: "Structures, alternative splicing, and neurexin binding of multiple neuroligins." in: The Journal of biological chemistry, Vol. 271, Issue 5, pp. 2676-82, 1996 (PubMed).