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G6PD (AA 308-320) Antikörper

Antigen

G6PD

Epitope
Alternativen

AA 308-320

Klonalität Polyklonal
Wirt
Alternativen

Ziege

Reaktivität
Alternativen

Human

Applikation
Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC), Enzyme Immunoassay (EIA)
1 Publikation vorhanden
Produktnummer ABIN190766
Menge 100µg  (100µg/200µl)
Preis 286,00 €   Zzgl. Versandkosten €40,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 Werktagen

Produktbeschreibung

Gen-ID 2539, 14381 (mouse), 24377 (rat)
Swiss-Prot NP_000393.4
Immunogen Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1.
Kreuzreaktivität Human, Maus, Ratte (Rattus), Hund
Format Liquid
Grade Serum-free
Beschreibung Official Symbol: G6PD
Spezifität This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1). Human, Mouse, Rat, Dog

Anwendungen

Applikationshinweise Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1µg/ml. IHC: In paraffin embedded Human Testis shows strong cytoplasm staining in spermatogonia. Recommended concentration, 2-4µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Konzentration 100µg/200µl
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Lagerung Aliquot and store at -20°C. Minimize freezing and thawing.
Beschränkungen Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen Guindo, Fairhurst, Doumbo et al.: "X-linked G6PD deficiency protects hemizygous males but not heterozygous females against severe malaria." in: PLoS medicine, Vol. 4, Issue 3, pp. e66, 2007 (PubMed).