GLU I (PR-2) Antikörper

Antigen: GLU I (PR-2)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Tabak (Nicotiana tabacum), Populus

Applikation: Western Blot (WB), Immunolokalisation (IL)

Produktnummer: ABIN190719

Produktbeschreibung

Swiss-Prot: Q42944

Immunogen: purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of amixture of closely related polypeptides encoded by a family of GLU I genescomprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLBP27666 derived from the tomentosiformis ancestor of tobacco,and homeologous recombinants (Sperisen et al., 1991). Mature GLU I isprocessed from a pre-pro-polypeptide (Shinshi et al., 1988).

Format: Lyophilized

Beschreibung: Pathogenesis-related (PR) proteins, are induced in response to the infection ofplants with microbial pathogens. Combinations of glucanase I and chitinase I arepotent inhibitors of fungal growth in vitro however precise mechanism of that isstill not known. Glucanase I and chitinase I contribute to defense against fungalinfection and are currently used as markers for innate immunity, and in particularthe ethylene/jasmonate signalling pathway in pathogenesis. Alternative names ofthe protein: basic beta-1,3-glucanase

Spezifität: Predicted reactivity: dicots including: Solanum lycopersicum, Solanum tuberosum. Not reactive in: Arabidopsis thaliana.

Anwendungen

Applikationshinweise: Recommended Dilution 8 ug/ml with standard ECL (WB). Additional Information: for more details on immunolocalization, please referr to Keefe et al (1990). Plant182: 43-51 This antibody can be used as a marker of vacuolar contents Keefe et al. (1990).The effect of ethylene on the cell-type-specific and intracellular localization ofβ-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Buffer: Affinity purified total IgG in PBS pH 7.4 (without Ca++)

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-GLU I (PR-2) antibody