Glutathione Reductase (GSR) Antikörper

Antigen: Glutathione Reductase (GSR)

Synonyme: GSR, GR, ATGR2, EMB2360, GLUTATHIONE REDUCTASE, Gr1, Gr-1, AI325518, D8Ertd238e, MGC78522, DTR, DmTrxR-1, TrxR, TrxR-1, Trxr1, anon-WO03040301.185, anon-WO03040301.187, l(1)G0154, l(1)G0379, l(1)G0477, l(1)G0481, DmelCG2151, CG2151

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Tabak (Nicotiana tabacum), Kartoffel (Solanum tuberosum), Maize/Corn (Zea mays)

Applikation: Western Blot (WB), Immunolokalisation (IL)

Produktnummer: ABIN190699

Produktbeschreibung

Weitere Bezeichnung: Glutathione reductase (GR)

Immunogen: maltose binding protein (MBP) fusion of Zea mays GR, O64409

Format: Lyophilized

Beschreibung: Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for plantprotection against environmental stress. It functions in plant defense reactions inthe conversion of glutathione disulphide to reduced glutathione (GSH).

Spezifität: Predicted reactivity: dicots including: Arabidopsis thaliana, Glycine max, Pisum sativum, monocotsincluding: Hordeum vulgare, Oryza sativa, trees: Populus balsamifera.

Synonyme: GSR, GR, ATGR2, EMB2360, GLUTATHIONE REDUCTASE, Gr1, Gr-1, AI325518, D8Ertd238e, MGC78522, DTR, DmTrxR-1, TrxR, TrxR-1, Trxr1, anon-WO03040301.185, anon-WO03040301.187, l(1)G0154, l(1)G0379, l(1)G0477, l(1)G0481, DmelCG2151, CG2151

Anwendungen

Applikationshinweise: Recommended Dilution 1: 5000 with standard ECL (WB), needs to be optimised.

Konzentration: total IgG concentration is 7 µg/ µl

Buffer: total IgG in PBS pH 7.4

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

10 ug of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB, (2) Nicotiana tabaccum leaf extracted with PEB, (3) Zea mays extracted with PEB, (4) Hordeum vulgare leaf extracted with PEB, (5) Physcomitrella patens total cell extracted with PEB, (6) Chlamydomonas reinhardtii total cell extracted with PEB, (7) Synochocystis elongatus total cell extracted with PEB, extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked in 2 % low fat dry milk in TBS-T (0.1 % Tween 20) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:30 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 30 seconds with WEST PICO reagent according the manufacturers instructions.

Publikationen

Publikationen: Mittova, Theodoulou, Kiddle et al.: "Coordinate induction of glutathione biosynthesis and glutathione-metabolizing enzymes is correlated with salt tolerance in tomato." in: FEBS letters, Vol. 554, Issue 3, pp. 417-21, 2003 (PubMed).

Sobrino-Plata, Ortega-Villasante, Flores-Cuaceres et al.: "Differential alterations of antioxidant defenses as bioindicators of mercury and cadmium toxicity in alfalfa." in: Chemosphere, Vol. 77, Issue 7, pp. 946-54, 2009 (PubMed).