Heat Shock Protein 70 (HSP70) (HSPA4) Antikörper

Antigen: Heat Shock Protein 70 (HSP70) (HSPA4)

Klonalität: Monoklonal (W27)

Wirt: Maus

Reaktivität: Rind (Kuh), Human, Affe

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN187606

Produktbeschreibung

Weitere Bezeichnung: Heat shock protein 70 / HSP70

Immunogen: Hsp70 protein from HeLa cells.Produced by fusion between BALB/c spleno-cytes and mouse myeloma NS-1 cells.

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: W27

Beschreibung: A wide variety of environmental and patho-physiological stress conditions trigger the synthesis of a family of proteins known as heat shock proteins, more appropriately called stress response proteins. Many heat shock proteins, including members of the Ssp70 family, are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation and secretion. Hsp70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other unknown proteins. Also, Hsp70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs and other damaging conditions.

Spezifität: This clone recognizes both the constitutive (Hsp73) and inducible (Hsp72) forms of Hsp70. Species Reactivity: Reacts with Human, Monkey and Bovine. Other species not tested. Positive Control: BT474 or HeLa cells. Breast carcinoma.

Anwendungen

Applikationshinweise: Flow Cytometry Immunofluorescence (Not assessed) Immunoprecipitation: Use at 2 µg/mg protein lysate. Use Protein A. Western blotting: 0.5-1 µg/ml for 2 hrs. at RT Immunohistology: Formalin/paraffin - Use at 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. The optimal dilution for a specific application should be determined by the researcher.

Buffer: Clone W27 is a monoclonal antibody against Heat Shock Factor 2. It is available at a concentration of 100ug/500ul in 10 mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Purified from ascites fluid by Protein A chromatography.

Lagerung: Stable for 24 months when stored at 2-8 °C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Heat Shock Protein 70 (HSP70) (HSPA4) antibody

anti-Heat Shock Protein 70 (HSP70) (HSPA4) antibody (Image 2)