Protein Interacting with PRKCA 1 (PICK1) Antikörper

Antigen: Protein Interacting with PRKCA 1 (PICK1)

Synonyme: PICK, PRKCABP, MGC15204, Prkcabp, prkcabp, MGC131295, PICK1, DKFZp470L2127

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185725

Produktbeschreibung

Weitere Bezeichnung: PICK1

Gen-ID: 9463, 18693 (mouse), 84591 (rat)

Immunogen: C-HYNKLQADPKQ

Format: Affinity-purified

Beschreibung: All names and symbols: PICK1, protein interacting with PRKCA 1, MGC15204, PRKCABP , OTTHUMP00000028509, PRKCA-binding protein, protein interacting with C kinase 1, protein kinase C, alpha binding protein. Official Symbol: PICK1. RefSeq number(s): NP_036539.1, NP_001034672.1, NP_001034673.1

Spezifität: All three variants represent the same protein. Human, Mouse, Rat, Dog 415 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in mouse and rat brain lysates (calculated MW of 46.6kDa according to NP_036539.1, NP_001034672.1and NP_001034673.1). Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Signaltransduktion, Tyrosinkinasen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185725 (1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Steinberg, Takamiya, Shen et al.: "Targeted in vivo mutations of the AMPA receptor subunit GluR2 and its interacting protein PICK1 eliminate cerebellar long-term depression." in: Neuron, Vol. 49, Issue 6, pp. 845-60, 2006 (PubMed).