Lipin 3 (LPIN3) Antikörper

Antigen: Lipin 3 (LPIN3)

Synonyme: SMP2, LIPN3L, dJ450M14.2, dJ450M14.3, dJ620E11.2, AA438110, AV236139, KIAA4023, MGC143991, MGC143992, mKIAA4023, 9130206L11Rik, MGC109016, MGC82840, LPIN3, DKFZp469D075

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Ziege

Applikation: ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN185722

Produktbeschreibung

Weitere Bezeichnung: Lipin 3

Gen-ID: 64900

Immunogen: C-KPKQKEDAVATD

Format: Affinity-purified

Beschreibung: All names and symbols: LPIN3, lipin 3 , RP4-620E11.3, LIPN3L, SMP2, dJ450M14.2, dJ450M14.3, dJ620E11.2 , lipin 3-like. Official Symbol: LPIN3. RefSeq number(s): NP_075047.1

Spezifität: Human 851 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Signaltransduktion, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Santos-Rosa, Leung, Grimsey et al.: "The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth." in: The EMBO journal, Vol. 24, Issue 11, pp. 1931-41, 2005 (PubMed).