Transcription Factor Like Protein 5 (TCFL5) Antikörper

Antigen: Transcription Factor Like Protein 5 (TCFL5)

Synonyme: CHA, Figlb, E2BP-1, MGC46135, AV260129, MGC118431, TCFL5

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN185720

Produktbeschreibung

Weitere Bezeichnung: CHA / TCFL5

Gen-ID: 10732, 277353 (mouse)

Immunogen: C-KYIQERHGDSLKKE

Format: Affinity-purified

Beschreibung: All names and symbols: TCFL5, CHA, transcription factor-like 5 (basic helix-loop-helix), E2BP-1, Figlb, MGC46135 , HPV-16 E2 binding protein 1, transcription factor-like 5 protein, Cha transcription factor. Official Symbol: TCFL5. RefSeq number(s): NP_006593.2

Spezifität: Human, Mouse, Rat, Dog 500 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185720 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Siep, Sleddens-Linkels, Mulders et al.: "Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis." in: Nucleic acids research, Vol. 32, Issue 21, pp. 6425-36, 2004 (PubMed).