SECIS Binding Protein 2 (SECISBP2) Antikörper

Antigen: SECIS Binding Protein 2 (SECISBP2)

Synonyme: SBP2, DKFZp686C09169, CG7066, DmelCG7066, 2210413N07Rik, 2810012K13Rik, Sbp2, DKFZp459F1026

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (IHC)

Produktnummer: ABIN185714

Produktbeschreibung

Weitere Bezeichnung: SBP2

Gen-ID: 79048

Immunogen: C-KERQERKQRLQEN

Format: Purified

Beschreibung: All names and symbols: SBP2 , RP11-89K14.1, SECISBP2, SECIS binding protein 2 , DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2. Official Symbol: SECISBP2. RefSeq number(s): NP_076982.3

Spezifität: Human, Dog 854 aa

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul,26(13):4895-910, PMID: 16782878). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3µg/ml. This antibody has also been used successfully for Immunoprecipitation.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN185714 staining of HFF cell lysate with B) 100nM siRNA or A) control siRNA. Detected by chemiluminescence. Data kindly provided by Laura Papp and Kum Kum Khanna, Brisbane, Australia.

ABIN185714 (3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publikationen

Publikationen: Papp, Lu, Striebel et al.: "The redox state of SECIS binding protein 2 controls its localization and selenocysteine incorporation function." in: Molecular and cellular biology, Vol. 26, Issue 13, pp. 4895-910, 2006 (PubMed).