TSPO Antikörper (C-Term)

Produktnummer ABIN185697

Der Ziege Polyklonal anti-TSPO Antikörper (ABIN185697) detektiert spezifisch TSPO in WB, IHC, ELISA, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TSPO Antikörper (C-Term) (ABIN185697)

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TSPO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-TSPO Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: TSPO / PBR (isoform)

Sequenz: RDNHGWRGGR RLPE

Spezifität: This antibody is expected to recognise one isoform (NP_000705.2) only.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDNHGWRGGRRLPE, from the C Terminus of the protein sequence according to NP_000705.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Colon shows strong cytoplasm staining of absorptive cells. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 17 kDa band observed in Human Breast cancer lysates and approx 16 kDa in Human Ovary and Skin lysates, and in lysates of cell line A431 (calculated MW of 18.8 kDa according to NP_000705.2).These molecular weights are routinely observed

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Andere Bezeichnung: TSPO

Hintergrund: TSPO, PBR, translocator protein (18 kDa) , BZRP, DBI, IBP, MBR, PKBS, PTBR, mDRC, pk18 , OTTHUMP00000028891, benzodiazapine receptor (peripheral), benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor, peripheral benzodiazepine rece

Gen-ID: 706

NCBI Accession: NP_000705

Pathways: Cellular Response to Molecule of Bacterial Origin