GTF2IRD1 Antikörper (Internal Region)

Produktnummer ABIN185681

Der Ziege Polyklonal anti-GTF2IRD1 Antikörper wird verwendet zum Nachweis von GTF2IRD1 in Proben von Human. Er wurde validiert für WB, ELISA, IF und FACS.

Kurzübersicht für GTF2IRD1 Antikörper (Internal Region) (ABIN185681)

Target: GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GTF2IRD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-GTF2IRD1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: GTF2IRD1

Sequenz: NKFTKDTTKL EPAS

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKFTKDTTKLEPAS, from the internal region of the protein sequence according to NP_057412.1, NP_005676.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 110 kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 106 kDa according to NP_057412.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GTF2IRD1

Target: GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))

Andere Bezeichnung: GTF2IRD1

Hintergrund: GTF2IRD1, GTF2I repeat domain containing 1 , HGNC:4661, CREAM1, GTF3, MUSTRD1, RBAP2, WBSCR11, WBSCR12, hMusTRD1alpha1 , GTF2I repeat domain-containing 1, Williams-Beuren syndrome chromosome region 11, general transcription factor 3, muscle TFII-I repeat

Gen-ID: 9569, 57080, 246770

NCBI Accession: NP_057412, NP_005676